Standard parameter

Measurement of standard parameters

The laboratories for environmental analysis at the Suderburg campus are used for ongoing research projects and student teaching, as well as for processing external analysis orders. The laboratory equipment enables employees to determine standard parameters such as pH value, conductivity, COD, NH 4-N, TKN and others. In addition, various laboratory devices are available for elemental and trace analysis to determine PAHs, permanent gases, CHCs, PCBs and aromatics. The laboratory for molecular biology deserves special mention.

Other equipment includes several models of technical systems on a laboratory scale. Tests can be carried out on digested sludge treatment and also sedimentation tests in a sedimentation tank. Furthermore, a laboratory wastewater treatment plant with denitrification stage, oxygen input control and continuous sludge recirculation is available for determining biodegradability.

  • Photometer for measuring cuvette tests (HachLange and Macherey&Nagel) with the corresponding digestion blocks

labot-standard1

  • Kjeldahl digestion block and distillation for the determination of NH 4-N, TKN and NOx

labot-standard2

  • Element analysis with the AAS Analyst 200 and ICP-OES Optima 8000 devices

labot-standard3

  • Various laboratory devices (GC 4 Autosystem with ECD for GC-MS, GC 1 Autosystem XL with ECD, GC 2 Autosystem with FID/WLD, GC 3 Autosystem XL with FID) for the determination of permanent gases, CHCs, PCBs and aromatics

labot-standard4

  • Rotary evaporator (HB 10, RV 10 digital, RV 10), spectrometer (UV/VIS Lambda 25), ultrapure water system (Barnstead Smart 2 Pure), HPLC (Dionex Ultimate 3000), GC-MS-MS and pyrolysis and ultrasonic bath

labot-standard5

Molecular biology

Molecular Biology

 

The laboratory for environmental analysis at the Suderburg campus was expanded in August 2022 to include a laboratory for molecular biology. The centerpiece of the laboratory equipment is the digital droplet PCR (ddPCR) from Bio Rad, consisting of a droplet generator, sealer, two thermal cyclers and the reader. In contrast to standard qPCR, ddPCR enables the exact (absolute) quantity determination of DNA sequences. The ddPCR can measure up to five target genes simultaneously from one sample. The "NanoDrop One" spectrophotometer from Fisher Scientific is used to determine RNA and DNA concentrations.

Water and sediment samples from wastewater and rainwater channels as well as surface waters are analyzed with regard to their contamination with pathogenic bacteria and viruses. Complex pre-treatment steps for nucleic acid extraction and purification are therefore necessary. Pressure filtration units and a cooling centrifuge are available for this purpose. The samples are stored professionally in an ultra-low temperature freezer.

The measurement results generated form the data basis for the development of forecasting tools and the creation of simulation models as part of the CHH's research projects. Current topics include the PRIUS project for the evaluation of pathogenic germs in rainwater sewers and sewer detectives for the detection of multi-resistant pathogens.

Labor-molekularbiologie

Figure 1: Overview of the microbiology laboratory

 

Labor-molekularbiologie2

Figure 2: ddPCR - droplet generator, sealer and thermal cycler from BioRad

 

Labor-molekularbiologie3Figure 3: ddPCR reader from BioRad

 

to top
Print